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KMID : 0613820070170020272
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Journal of Life Science
2007 Volume.17 No. 2 p.272 ~ p.278
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Isolation of Pseudoalteromonas sp. HJ 47 from Deep Sea Water of East Sea and Characterization of its Extracellular Protease
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Cha In-Tae
Lim Hyung-Joon
Roh Dong-Hyun
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Abstract
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Proteases are enzymes that break peptide bonds between amino acids of other proteins and occupy a crucial position with respect to their applications in both physiological and commercial fields. In order to screen new source of protease, bacteria producing extracellular proteases at low temperature were isolated from deep sea water of East Sea, Korea. A bacterium showing the best growth rate and production of an extracellular protease at low temperature was designated HJ 47. The DNA sequence analysis of the 16S rRNA gene, phenotypic tests and morphology led to the placement of this organism in the genus Pseudoalteromonas. Although maximal growth was observed at 37℃, enzyme production per culture time was maximum at 20℃. At this temperature, extracellluar protease production was detected from the end of the exponential phage to stationary phase, and maximal at 15 hours after initial production. The optimum temperature and pH of the protease were found to be 35℃ and 8.
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KEYWORD
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extracellular protease, Pseudoalteromonas sp, psychrotolerant strain
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